Bioinformatics@HSL: BBT Workshop Descriptions

The Basic Bioinformatics Tools Workshop Series provides seminar and hands-on computer workshops on bioinformatics tools and resources to faculty, staff and students of UNC-Chapel Hill. This workshop series is sponsored by the UNC Health Sciences Library in partnership with the UNC Bioinformatics and Analytics Research Collaborative (BARC).

Below are descriptions of workshops that have been regularly offered in past semesters.

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Introduction to the UNIX Shell: A Six Workshop Sequence

The six, one-hour Introduction to the UNIX Shell workshops below are a chained sequence of workshops to introduce participants to the UNIX command line, basic commands, navigating the file system, setting up bioinformatics projects, and introductory scripting. Each class will build upon the prior class and each session is a prerequisite for the next class in the six-workshop UNIX Shell sequence.

The six workshops in the UNIX Shell sequence are in turn prerequisites for the six, one-hour Introduction to Bioinformatics Basics workshops listed below which follow in the semester-long workshop series.

NOTE: These classes can fill quickly. Seats that open up when registrants cancel/withdraw will be offered to those on the waitlist in order of registration. REGISTER ONLY if you plan to ATTEND the live workshop and are willing to review any missed prerequisite content.

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1. UNIX Shell Basics 1   Register

Time: Monday 9/15/2025, 2:00pm - 3:00pm

Instructor: Tristan De Buysscher, Bioinformatics Scientist, Bioinformatics and Analytics Research Collaborative (BARC)
Location: HSL 329

Account Requirement: You will need an account on the “Longleaf” Linux cluster managed by ITS Research Computing. Follow instructions for requesting a “Longleaf” account on the ITS Research Computing website: https://help.rc.unc.edu/request-a-cluster-account/. Please submit your account request at least 3 days before class. It generally takes 24-48 hours for account creation.

Prerequisites: No prior knowledge of UNIX is required.

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2. UNIX Shell Basics 2   Register

Time: Wednesday 9/17/2025, 2:00pm - 3:00pm

Instructor: Tristan De Buysscher, Bioinformatics Scientist, Bioinformatics and Analytics Research Collaborative (BARC)
Location: HSL 329

Account Requirement: See UNIX Shell Basics 1 Account Requirement above.

Prerequisites: UNIX Shell Basics 1 is a prerequisite for this UNIX Shell Basics 2 workshop.

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3. Working with Files   Register

Time: Monday 9/22/2025, 2:00pm - 3:00pm

Instructor: Tristan De Buysscher, Bioinformatics Scientist, Bioinformatics and Analytics Research Collaborative (BARC)
Location: HSL 329

Account Requirement: See UNIX Shell Basics 1 Account Requirement above.

Prerequisites: UNIX Shell Basics 1 and UNIX Shell Basics 2 are prerequisites for this Working with Files workshop.

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4. Useful Tools and Pipes   Register

Time: Wednesday 9/24/2025, 2:00pm - 3:00pm

Instructor: Tristan De Buysscher, Bioinformatics Scientist, Bioinformatics and Analytics Research Collaborative (BARC)
Location: HSL 329

Account Requirement: See UNIX Shell Basics 1 Account Requirement above.

Prerequisites: UNIX Shell Basics 1, UNIX Shell Basics 2, and Working with Files are prerequisites for this Useful Tools and Pipes workshop.

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5. Loops and Scripts   Register

Time: Monday 9/29/2025, 2:00pm - 3:00pm

Instructor: Tristan De Buysscher, Bioinformatics Scientist, Bioinformatics and Analytics Research Collaborative (BARC)
Location: HSL 329

Account Requirement: See UNIX Shell Basics 1 Account Requirement above.

Prerequisites: UNIX Shell Basics 1, UNIX Shell Basics 2, Working with Files, and Useful Tools and Pipes are prerequisites for this Loops and Scripts workshop.

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6. Introduction to Slurm   Register

Time: Wednesday 10/1/2025, 2:00pm - 3:00pm

Instructor: Tristan De Buysscher, Bioinformatics Scientist, Bioinformatics and Analytics Research Collaborative (BARC)
Location: HSL 329

Account Requirement: See UNIX Shell Basics 1 Account Requirement above.

Prerequisites: UNIX Shell Basics 1, UNIX Shell Basics 2, Working with Files, Useful Tools and Pipes, and Loops and Scripts are prerequisites for this Introduction to Slurm workshop.

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Introduction to Bioinformatics Basics: A Six Workshop Sequence

NOTE: Registration for this sequence of workshops will open in late September.

This six workshop sequence will cover bioinformatics data types, basic quality control of short read data including assessing quality and filtering data, and basic bioinformatics methods including how to align data to a reference genome and call variants. Time permitting, some scripting will be covered as well.

The six workshops in UNIX Shell sequence above are prerequisites for the six-workshop Introduction to Bioinformatics Basics sequence. Additionally, each class in the Introduction to Bioinformatics Basics sequence will build upon the prior class and each session is a prerequisite for the next class in the sequence.

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7. Intro to Bioinformatics Basics: Quality Control and Trimming

Time:  Monday 10/6/2025, 2:00pm - 3:00pm

Instructor: Tristan De Buysscher, Bioinformatics Scientist, Bioinformatics and Analytics Research Collaborative (BARC)
Location: HSL 329

Account Requirement: See UNIX Shell Basics 1 Account Requirement above.

Prerequisites: The six workshops in UNIX Shell sequence above (UNIX Shell Basics 1, UNIX Shell Basics 2, Working with Files, Useful Tools and Pipes, Loops and Scripts, and Introduction to Slurm) are prerequisites for this Quality Control and Trimming workshop.

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8. Intro to Bioinformatics Basics: Aligning Reads

Time:  Wednesday 10/8/2025, 2:00pm - 3:00pm

Instructor: Tristan De Buysscher, Bioinformatics Scientist, Bioinformatics and Analytics Research Collaborative (BARC)
Location: HSL 329

Account Requirement: See UNIX Shell Basics 1 Account Requirement above.

Prerequisites:

• The six workshops in UNIX Shell sequence above
• Intro to Bioinformatics Basics: Quality Control and Trimming

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9. Differential Expression Analysis: Intro and Counting

Time:  Monday 10/13/2025, 2:00pm - 3:00pm

Instructor: Tristan De Buysscher, Bioinformatics Scientist, Bioinformatics and Analytics Research Collaborative (BARC)
Location: HSL 329

Account Requirement: See UNIX Shell Basics 1 Account Requirement above.

Prerequisites:

• The six workshops in UNIX Shell sequence above
• Intro to Bioinformatics Basics: Quality Control and Trimming
• Intro to Bioinformatics Basics: Aligning Reads

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10. Differential Expression Analysis: DESeq Basics

Time:  Wednesday 10/15/2025, 2:00pm - 3:00pm

Instructor: Tristan De Buysscher, Bioinformatics Scientist, Bioinformatics and Analytics Research Collaborative (BARC)
Location: HSL 329

Account Requirement: See UNIX Shell Basics 1 Account Requirement above.

Prerequisites:

• The six workshops in UNIX Shell sequence above
• Intro to Bioinformatics Basics: Quality Control and Trimming
• Intro to Bioinformatics Basics: Aligning Reads
• Differential Expression Analysis: Intro and Counting

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11. Differential Expression Analysis: Intermediate DESeq & RStudio

Time:  Monday 10/20/2025, 2:00pm - 3:00pm

Instructor: Tristan De Buysscher, Bioinformatics Scientist, Bioinformatics and Analytics Research Collaborative (BARC)
Location: HSL 329

Account Requirement: See UNIX Shell Basics 1 Account Requirement above.

Prerequisites:

• The six workshops in UNIX Shell sequence above
• Intro to Bioinformatics Basics: Quality Control and Trimming
• Intro to Bioinformatics Basics: Aligning Reads
• Differential Expression Analysis: Intro and Counting
• Differential Expression Analysis: DESeq Basics

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12. Simple Multiomics

Time:  Wednesday 10/22/2025, 2:00pm - 3:00pm

Instructor: Tristan De Buysscher, Bioinformatics Scientist, Bioinformatics and Analytics Research Collaborative (BARC)
Location: HSL 329

Account Requirement: See UNIX Shell Basics 1 Account Requirement above.

Prerequisites:

• The six workshops in UNIX Shell sequence above
• Intro to Bioinformatics Basics: Quality Control and Trimming
• Intro to Bioinformatics Basics: Aligning Reads
• Differential Expression Analysis: Intro and Counting
• Differential Expression Analysis: DESeq Basics
• Differential Expression Analysis: DESeq Basics
• Differential Expression Analysis: Intermediate DESeq & RStudio

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